Rotate the dark box in the Use the FXDX dark box or the NFX dark arrow direction to the limit to attach the dark box for 35 mm film photomicrography. Page 20 'Electrical Specifications' on page 72 and different ways depending on the type of a the Nikon 12VW lamphouse for U-epi projection Jense to be used.
Fit the socket sleeve into the lamphouse until it reaches the limit. Diopter Adjustment 1 Push the mirror block changeover lever to put the brightfield mirror block with B. Page 23 6 Turn the zoom dial to select lx magnification. Figure right 7 Rotate the coarse focus knob to lower the revolving nosepiece to the limit. Rotate the nosepiece to put the lOx objective in the optical path. Rotate the stage movement knobs to locate the center of the opening of the stage ring almost right above the top of that objective.
Page Interpupillary Distance Adjustment 11 Rotate the diopter correction ring of the left eyepiece to focus on the sample through this eyepiece. It also maintain the mechanical tube length correctly and helps high quality objectives to fully exhibit their performance.
When 12VW halogen lamps is used, proceed to the next section since it doesn't need centering. Page Centering Of Field Diaphragm 12 Loosen the eyepiece tube clamp screw to turn the eyepiece tube to the right. Tighten the clamp screw to fix the eyepiece tube.
If the operator can easily reach the lamphouse from the front of the microscope while looking into the eyepiece facing front, it is not necessary to change the direction of the eyepiece tube. Page 27 9 Insert each of the two centering tools into the screw holes for field diaphragm centering.
Rotate the centering tools to center the field diaphragm until the the field diaphragm image and the eyepiece viewfield are concentric. Page Centering Of Aperture Diaphragm 5. Centering of Aperture Diaphragm 1 Push the mirror block changeover lever to put the brightfield mirror block in the optical path.
Page 29 12 Stop down the aperture diaphragm to minimum. The diaphragm image then become visible on the objective's exit pupil. Rotate the centering tools to center the aperture diaphragm until the aperture diaphragm image and the exit pupil are concentric. Objecti ve's exit pu pi l Aperture diaphragm image Page 31 I. Page 32 9 Switch to the desired objective and focus on the sample. However, the top lens of each of these objectives slightly protrudes beyond the metal rim around it.
Page 33 10 Adjust the brightness with ND filters and the lamp intensity control dial. The fi e ld diaphragm restricts the illumination light only to the sample's area to be illuminated for observation. Page Darkfield Microscopy 2. Darkfield Microscopy I Pull the mirror block changeover lever to the click stop to put the darkfield mirror block with D. Page 35 Darkfield Microscopy" written below 10 Switch to the desired objective and focus on the sample.
Page 36 9 Switch to the desired objective and focus on the sample. Page 37 11 Put polarizer and analyzer in the optical path. Push the prism slider until it reaches the limit.
Rotate the nosepiece to bring the prism in the optical path. Nomarski prisms and objectives are to be used in pairs. Page 38 10 Switch to the desired objective to focus on the sample. Page 39 11 Tum the aperture and field diaphragm dials to open or stop down these two diaphragms properly. Keep the first order red compensator out of the optical path. Filters The table below shows the functions of filters, their names, what they are used for, and where they are used.
What they are Where they are Functions Names used for. When the use of this filter is not needed, pull this lever to the limit. Page Aperture Diaphragm 2. The objective's exit pupil is visible there. Page Analyzer Slider 5. Analyzer Slider The analyzer slider is used in combination with the polarizer and Nomarski prisms in simplified polarization, sensitive polarization and differential interference contrast microscopy.
Page 46 7. The illuminator of this microscope can accommodate two of these mirror or filter blocks at one time. Page 47 8. Page Eyepiece Tube 9. Eyepiece Tube The right eyepiece sleeve of the binocular eyepiece tube contains a mechanism capable of keeping an eyepiece in it from being rotated during interpupillary distance adjustment.
Insert the eyepiece with a built-in mask with EPM indiGation into this eyepiece sleeve. Page 49 I I. A sample placed on the stage can be focused by rotating these focus knobs. Figure right shows the relation between their rotation and the nosepiece movement. Page Rectangular Mechanical Stage The operator can therefore angle the handle to positions convenient for him to move the stage.
He also can shift his hand quite easily from the coarse focus and fine focus knobs to the stage movement knobs and vice versa. Page Grain Reticle Adjust its position in the direction in which it slides until the pattern is located in the center of the viewfield. Page 52 Turn the micrometer changeover dial of the slider to switch to the micrometer which suits the magnification of the objective in the optical path.
Page Optical System Carefully rotate the revolving nosepiece to which these high magnification objectives have been mounted. If it does not, re-load the film correctly. If this LED is It is not necessary to keep depressing or sliding the switch. Page 57 2. Microscope: Nikon Epiphot with 20X objective Mag. The magnification was set at X for a calibration factor of 0. Installing and Replacing Lamps. Use only the specified mercury lamps p3. For details on handling lamps, please see the instruction manual supplied Refer to page See manual.
Measurements are performed on solid grains. Figure 1: Original image. Figure 2: Grain outlines overlaid against the original image. Image Analysis. System: Clemex Vision Software. Microscope : Nikon Epiphot Sep 30, Magnification Equations. Feb 7, The measurement of the properties and behaviour of material is of fundamental importance in modern engineering.
In recent years a breakthrough method for measuring surface deformation has been represented by digital image correlation. To determine local deformations in a material, Jul 1, The results of the automatic method are contrasted with the manual measurements for one All Rights Reserved.
Designed by Templatic. Home Copyright Privacy Contact. Nikon Epiphot Manual. Sponsored High Speed Downloads. Nikon TE inverted microscope Thank you for purchasing the Nikon products. Manual Thank you for purchasing a Nikon product. Ti-E manual. New Datasheet Guidelines Prior Scientific has introduced a new focus only control system for modern microscopes. Inverted Microscope Although Nikon products are designed to provide you with the utmost safety during use, incorrect usage or disregard of the instructions can cause personal injury or property damage and will lead to the forfeiture of all claims against warranty.
Alpha grain size in titanium Nikon Epiphot with a 20x objective. Thermal spray coating thickness Equipment Used. Procedure for changing lamp in Nikon microscope Installing and Replacing Lamps. Grain size in anodized aluminum spread to their full size using zone binary tool. Microscope Adapter Catalog Sep 30, Microcontact printing on metallic surfaces for optical deformation Feb 7, Popular Documents building the perfect zombie bug out bag user manual honda calculus of a single variable 6th edition solution manual geoinformatics for intelligent transportation lecture notes in geoinformation and cartography b w dm 4 bowers wilkins crossover diagram components caterpillar gp25 manual peugeot service repair manual pdf 87 97 the nondesigners indesign book renal and adrenal tumors biology and management slow burn box set 1 3 slow burn zombie apocalypse series turfgrass science and management annals of tropical medicine and parasitology bmw krs service repair manual evergreen 9th science guide the cras guide to monitoring clinical research third edition mcsa windows server complete study guide exams 70 70 70 and 70 70 guide honda xlr manual.
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